GSM8670270: NBM6_CD14; Homo sapiens; RNA-Seq - SRA

SRX26997134: GSM8670270: NBM6_CD14; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 30.6M spots, 9.2G bases, 2.8Gb downloads

External Id: GSM8670270_r1

Submitted by: Washington University School of Medicine

Study: RSK1 is an exploitable dependency in myeloid malignancies [CD14_RNAseq]

PRJNA1195077 • SRP549821 • All experiments • All runs

show Abstracthide Abstract

RSK1 is an exploitable dependency in myeloid malignancies Overall design: Here, we establish a comprehensive RNA-seq dataset of CD14+ cells encompassing 68 patient samples across the spectrum of myeloproliferative neoplasms with normal donor controls. CD14+ cells were bead-enriched from the CD34- sorted fraction from MPN PBMCs and normal healthy donor (bone marrow) to assess gene expression patterns.

Sample: NBM6_CD14

SAMN45208117 • SRS23462409 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM8670270

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: The CD34- cell fraction was then enriched for CD14+ cells with bead purification.Total RNA from cell lines was extracted with RNeasy Mini Kit (Qiagen, Hilden, Germany) with DNAse treatment following the manufacturer's protocol. Samples were then indexed, pooled, and sequenced by Illumina NovaSeq 6000 following standard Illumina protocol.

Runs: 1 run, 30.6M spots, 9.2G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR31633173	30,621,362	9.2G	2.8Gb	2024-12-06

ID:: 36460621

SRA

Sequence Read Archive

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